Stereology imagej4/1/2024 ![]() ![]() If you have thin sections (a couple of microns thick) you need to use pairs of thin sections (see Physical Disector for NvVref or Fractionator for the fractionator method in the chart above). To estimate number of objects in a three-dimensional region you need a three dimensional probe so that the leading edge of the particles can be identified. For the Fractionator, an extrapolation is done to arrive at an estimate (if you are working in one petri dish the ssf is one): For the Disector, a numerical density is calculated and multiplied by the reference area. The number of objects in a flat region, for instance at the bottom of a petri dish can be estimated by using single counting frames (in the chart above, Disector refers to the NvVref method and Fractionator uses the fractionator method). If you calculate a numerical density it is called the disector method, an example of the NvVref method. If you keep track of the volume fraction and extrapolate to get the estimate, it is called the fractionator method. This is because a unique point on the particle is counted, and a point looks the same from any direction. Tissue sections of any orientation, including a preferred orientation may be used. A very common example is the number of cells. The counting frame and systematic random sampling can be used to estimate the number of objects without bias. ![]()
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